EphB2 regulates contact-dependent and independent signalling to control platelet function

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Vaiyapuri, S. ORCID: https://orcid.org/0000-0002-6006-6517, Sage, T., Rana, R. H., Schenk, M. P., Ali, M. S., Unsworth, A. J., Jones, C. I. ORCID: https://orcid.org/0000-0001-7537-1509, Stainer, A. R., Kriek, N. K., Moraes, L. A. and Gibbins, J. ORCID: https://orcid.org/0000-0002-0372-5352 (2015) EphB2 regulates contact-dependent and independent signalling to control platelet function. Blood, 125 (4). pp. 720-730. ISSN 0006-4971 doi: 10.1182/blood-2014-06-585083

Abstract/Summary

The Eph kinases, EphA4 and EphB1 and their ligand, ephrinB1 have been previously reported to be present in platelets where they contribute to thrombus stability. While thrombus formation allows for Eph-ephrin engagement and bidirectional signalling, the importance specifically of Eph kinase or ephrin signalling in regulating platelet function remained unidentified. In the present study, a genetic approach was used in mice to establish the contribution of signalling orchestrated by the cytoplasmic domain of EphB2 (a newly discovered Eph kinase in platelets) in platelet activation and thrombus formation. We conclude that EphB2 signalling is involved in the regulation of thrombus formation and clot retraction. Furthermore, the cytoplasmic tail of this Eph kinase regulates initial platelet activation in a contact-independent manner in the absence of Eph-ephrin ligation between platelets. Together these data demonstrate that EphB2 signalling not only modulates platelet function within a thrombus but is also involved in the regulation of the function of isolated platelets in a contact-independent manner.

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Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/38637
Item Type	Article
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR) Life Sciences > School of Biological Sciences > Biomedical Sciences
Uncontrolled Keywords	platelets, thrombosis, haemostasis, eph kinase, ephrin, contact
Publisher	American Society of Hematology
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Funded Project

Funders:	British Heart Foundation Medical Research Council	The sponsoring bodies who contributed funding for the creation of this item. Example: NERC Example: The Royal Society of Chemistry A pick list of funders may appear as you type in the funder's name in full or as an acronym. Select a correct match to complete the field or type in a new entry in full. For new entries, the full name is preferred.
Projects:	The physiological importance and integration of receptor-mediated inhibitory mechanisms in platelets Funded by: British Heart Foundation (RG/09/011/28094 - £937,084) Local Lead (PI): Jon Gibbins 1 September 2010 - 31 August 2015 Study of the role of secreted platelet thiol isomerases in the regulation of platelet function, haemostasis and thrombosis Funded by: Medical Research Council (MR/J002666/1 - £1,175,779) Local Lead (PI): Jon Gibbins Project Lead: Jonathan Martin Gibbins 18 June 2012 - 17 June 2017 Study of the role of gap junctions and connexin hemichannels in the control of platlet function, haemostasis and thrombosis Funded by: British Heart Foundation (PG/11/125/29320 - £204,735) Local Lead (PI): Jon Gibbins 16 March 2012 - 15 March 2015	Click Add to select your project (received at Reading) from an autocomplete list.

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Date Deposited:	22 Dec 2014 17:22	Date item deposited into CentAUR
Last Modified:	23 Jun 2024 04:17	Date item last modified

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