Fully automated platelet differential interference contrast image analysis via deep learning

Kempster, C., Butler, G., Kuznecova, E., Taylor, K. A. ORCID: https://orcid.org/0000-0002-4599-7727, Kriek, N., Little, G., Sowa, M. A., Sage, T., Johnson, L. J. ORCID: https://orcid.org/0000-0002-0006-1511, Gibbins, J. M. ORCID: https://orcid.org/0000-0002-0372-5352 and Pollitt, A. Y. ORCID: https://orcid.org/0000-0001-8706-5154 (2022) Fully automated platelet differential interference contrast image analysis via deep learning. Scientific Reports, 12. 4614. ISSN 2045-2322 doi: 10.1038/s41598-022-08613-2

Abstract/Summary

Platelets mediate arterial thrombosis, a leading cause of myocardial infarction and stroke. During injury, platelets adhere and spread over exposed subendothelial matrix substrates of the damaged blood vessel wall. The mechanisms which govern platelet activation and their interaction with a range of substrates are therefore regularly investigated using platelet spreading assays. These assays often use differential interference contrast (DIC) microscopy to assess platelet morphology and analysis performed using manual annotation. Here, a convolutional neural network (CNN) allowed fully automated analysis of platelet spreading assays captured by DIC microscopy. The CNN was trained using 120 generalised training images. Increasing the number of training images increases the mean average precision of the CNN. The CNN performance was compared to six manual annotators. Significant variation was observed between annotators, highlighting bias when manual analysis is performed. The CNN effectively analysed platelet morphology when platelets spread over a range of substrates (CRP-XL, vWF and fibrinogen), in the presence and absence of inhibitors (dasatinib, ibrutinib and PRT-060318) and agonist (thrombin), with results consistent in quantifying spread platelet area which is comparable to published literature. The application of a CNN enables, for the first time, automated analysis of platelet spreading assays captured by DIC microscopy.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/104101
Identification Number/DOI	10.1038/s41598-022-08613-2
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	Nature Publishing Group
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