Identification of novel aphid-killing bacteria to protect plants

Paliwal, D. ORCID: https://orcid.org/0000-0002-6891-0778, Hamilton, A. J., Barrett, G. A. ORCID: https://orcid.org/0000-0003-1509-0179, Alberti, F., Van Emden, H., Monteil, C. L., Machuline, T. H., Nauen, R., Wagstaff, C. ORCID: https://orcid.org/0000-0001-9400-8641, Bass, C. and Jackson, R. W. (2022) Identification of novel aphid-killing bacteria to protect plants. Microbial Biotechnology, 15 (4). pp. 1203-1220. ISSN 1751-7915 doi: 10.1111/1751-7915.13902

Abstract/Summary

Aphids, including the peach-potato aphid, Myzus persicae, are major insect pests of agriculture and horticulture, and aphid control measures are limited. There is therefore an urgent need to develop alternative and more sustainable means of control. Recent studies have shown that environmental microbes have varying abilities to kill insects. We screened a range of environmental bacteria isolates for their abilities to kill target aphid species. Tests demonstrated the killing aptitude of these bacteria against six aphid genera (including Myzus persicae). No single bacterial strain was identified that was consistently toxic to insecticide-resistant aphid clones than susceptible clones, suggesting resistance to chemicals is not strongly correlated with bacterial challenge. Pseudomonas fluorescens PpR24 proved the most toxic to almost all aphid clones whilst exhibiting the ability to survive for over three weeks on three plant species at populations of 5-6 log CFU cm-2 leaf. Application of PpR24 to plants immediately prior to introducing aphids onto the plants led to a 68%, 57% and 69% reduction in aphid populations, after 21 days, on Capsicum annuum, Arabidopsis thaliana and Beta vulgaris respectively. Together, these findings provide new insights into aphid susceptibility to bacterial infection with the aim of utilizing bacteria as effective biocontrol agents.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/99812
Identification Number/DOI	10.1111/1751-7915.13902
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences Life Sciences > School of Biological Sciences > Ecology and Evolutionary Biology Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
Publisher	Wiley
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