Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa

Alcock, T. D., Thomas, C. L., Ó Lochlainn, S., Pongrac, P., Wilson, M., Moore, C., Reyt, G., Vogel-Mikuš,, K., Kelemen, M., Hayden, R., Wilson, L., Stephenson, P., Østergaard, L., Irwin, J. A., Hammond, J. P. ORCID: https://orcid.org/0000-0002-6241-3551, King, G. J., Salt, D. E., Graham, N. S., White, P. J. and Broadley, M. R. (2021) Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa. Plant Physiology, 186 (3). pp. 1616-1631. ISSN 1532-2548 doi: 10.1093/plphys/kiab150

Abstract/Summary

Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) mutant, braA.sgn3.a-1, which accumulates twice as much Mg and a third more Ca in its leaves. We mapped braA.sgn3.a to a single recessive locus using a forward ionomic screen of chemically mutagenized lines with subsequent backcrossing and linked-read sequencing of second back-crossed, second filial generation (BC2F2) segregants. Confocal imaging revealed a disrupted root endodermal diffusion barrier, consistent with SGN3 encoding a receptor-like kinase required for normal formation of Casparian strips, as reported in thale cress (Arabidopsis thaliana). Analysis of the spatial distribution of elements showed elevated extracellular Mg concentrations in leaves of braA.sgn3.a-1, hypothesized to result from preferential export of excessive Mg from cells to ensure suitable cellular concentrations. This work confirms a conserved role of SGN3 in controlling nutrient homeostasis in B. rapa, and reveals mechanisms by which plants are able to deal with perturbed shoot element concentrations resulting from a “leaky” root endodermal barrier. Characterization of variation in leaf Mg and Ca accumulation across a mutagenized population of B. rapa shows promise for using such populations in breeding programs to increase edible concentrations of essential human and animal nutrients.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/99261
Identification Number/DOI	10.1093/plphys/kiab150
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Publisher	Oxford University Press (OUP)
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