Characterization of microsatellite loci for the endangered cactus Echinocactus grusonii, and their cross-species utilization

Hardesty, B. D., Hughes, S. L., Rodriguez, V. M. and Hawkins, J. A. ORCID: https://orcid.org/0000-0002-9048-8016 (2008) Characterization of microsatellite loci for the endangered cactus Echinocactus grusonii, and their cross-species utilization. Molecular Ecology Resources, 8 (1). pp. 164-167. ISSN 1471-8278 doi: 10.1111/j.1471-8286.2007.01913.x

Abstract/Summary

Echinocactus grusonii is common in trade but critically endangered in its natural habitat. With the ultimate aim of developing a certification scheme to aid in the conservation of this species, we have isolated E. grusonii microsatellites from a nonenriched library. Fifty-seven sequences contained a microsatellite array, of which 12 were polymorphic among 30 individuals from a single wild population. All 12 microsatellite primer pairs amplified product in one or more species in a screen of 27 other cactus species.