Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli

Baba-Dikwa, A., Thompson, D., Spencer, N. J., Andrews, S. C. ORCID: https://orcid.org/0000-0003-4295-2686 and Watson, K. A. ORCID: https://orcid.org/0000-0002-9987-8539 (2008) Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 64. pp. 966-969. ISSN 1744-3091 doi: 10.1107/s1744309108029515

Abstract/Summary

The yncE gene of Escherichia coli encodes a predicted periplasmic protein of unknown function. The gene is de-repressed under iron restriction through the action of the global iron regulator Fur. This suggests a role in iron acquisition, which is supported by the presence of the adjacent yncD gene encoding a potential TonB-dependent outer-membrane transporter. Here, the preliminary crystallographic structure of YncE is reported, revealing that it consists of a seven-bladed beta-propeller which resembles the corresponding domain of the `surface-layer protein' of Methanosarcina mazei. A full structure determination is under way in order to provide insight into the function of this protein.