Assessment of the anti-pathogenic effects of condensed tannin extracts using scanning electron microscopy

Dakheel, M. M. ORCID: https://orcid.org/0000-0002-1938-8098, Kaur, A., Al-Mnaser, A. A., Mueller-Harvey, I., Woodward, M. J. and Rymer, C. ORCID: https://orcid.org/0000-0002-3535-4330 (2021) Assessment of the anti-pathogenic effects of condensed tannin extracts using scanning electron microscopy. Archives of Microbiology, 203. pp. 1555-1563. ISSN 0302-8933 doi: 10.1007/s00203-020-02147-9

Abstract/Summary

Two diferent types of condensed tannins (CTs), which were extracted and purifed from tilia (Tilia L.) and black locust (Robinia pseudoacacia), were studied and tested against two kinds of bacteria, including Gram-negative and Gram-positive, avian pathogenic E. coli (APEC) and Staphylococcus epidermidis (S. epidermidis) respectively, by minimal bactericidal concentrations (MBCs) and scanning electron microscopy (SEM). Both CT extracts were signifcantly efective (p≤0.05) at MBCs of 5–10 mg CT/ml against APEC (Gram-negative), and at 1.25–5 mg CT/ml on S. epidermidis (Gram-positive). This indicated that the CTs were more potent against the Gram-positive than the Gram-negative bacteria. Further, SEM revealed that CTs caused mainly morphological deformations of the bacterial cells and some conjoined cell growth.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/95545
Identification Number/DOI	10.1007/s00203-020-02147-9
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Animal Sciences > Animal, Dairy and Food Chain Sciences (ADFCS)- DO NOT USE
Publisher	Springer
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