Characterization of peptides and their assemblies

Hamley, I. W. ORCID: https://orcid.org/0000-0002-4549-0926 and Castelletto, V. (2020) Characterization of peptides and their assemblies. In: Guler, M. O. (ed.) Peptide-based Biomaterials. Royal Society of Chemistry, pp. 19-46. ISBN 9781788017299 doi: 10.1039/9781839161148-00019

Abstract/Summary

Methods to characterize the conformation and self-assembly or aggregation of peptides and peptide conjugates are reviewed. Spectroscopic methods to probe conformation, including circular dichroism, Fourier-transform infrared, Raman and NMR spectroscopy, are outlined. Aggregation can be detected by fluorescence probe and analytical ultracentrifugation methods, which are discussed. The analysis of structural information on assemblies from X-ray diffraction, light scattering, small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) is considered. Imaging methods are also summarized, including atomic force microscopy (AFM) and electron microscopy (SEM and TEM). Peptide gel mechanical analysis using rheometry is also discussed. Selected examples from the literature are used to highlight the applicability of the methods.

Item Type	Book or Report Section
URI	https://reading-clone.eprints-hosting.org/id/eprint/94690
Identification Number/DOI	10.1039/9781839161148-00019
Refereed	No
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	Royal Society of Chemistry
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