Development of a minimal KASP marker panel for distinguishing genotypes in apple collections

Winfield, M. O., Burridge, A., Ordidge, M. ORCID: https://orcid.org/0000-0003-0115-5218, Harper, H., Wilkinson, P., Thorogood, D., Copas, E., Edwards, K. and Barker, G. (2020) Development of a minimal KASP marker panel for distinguishing genotypes in apple collections. PLoS ONE, 15 (11). e0242940. ISSN 1932-6203 doi: 10.1371/journal.pone.0242940

Abstract/Summary

Accurate identification of named accessions in germplasm collections is extremely important, especially for vegetatively propagated crops which are expensive to maintain.  Thus, an inexpensive, reliable, and rapid genotyping method is essential because it avoids the need for laborious and time-consuming morphological comparisons.  Single Nucleotide Polymorphism (SNP) marker panels containing large numbers of SNPs have been developed for many crop species, but such panels are much too large for basic cultivar identification.  Here, we have identified a minimum set of SNP markers sufficient to distinguish apple cultivars held in the English and Welsh national collections providing a cheaper and automatable alternative to the markers currently used by the community.  We show that SNP genotyping with a small set of well selected markers is equally efficient as microsatellites for the identification of apple cultivars and has the added advantage of automation and reduced cost when screening large numbers of samples

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/94435
Identification Number/DOI	10.1371/journal.pone.0242940
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Publisher	Public Library of Science
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