Antifibrotic and regenerative effects of Treamid in pulmonary fibrosis

Skurikhin, E., Nebolsin, V., Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X, Ermakova, N., Pershina, O., Pakhomova, A., Krupin, V., Pan, E., Zhukova, M., Novikov, F., Sandrikina, L., Morozov, S., Kubatiev, A. and Dygai, A. (2020) Antifibrotic and regenerative effects of Treamid in pulmonary fibrosis. International Journal of Molecular Sciences, 21 (21). 8380. ISSN 1422-0067 doi: 10.3390/ijms21218380

Abstract/Summary

Idiopathic pulmonary fibrosis (IPF) is a chronic progressive disease characterized by interstitial fibrosis and progressive respiratory failure. Pirfenidone and nintedanib slow down but do not stop the progression of IPF. Thus, new compounds with high antifibrotic activity and simultaneously regenerative activity are an unmet clinical need. Recently, we showed that Treamid can help restoring the pancreas and testicular tissue in mice with metabolic disorders. We hypothesized that Treamid may be effective in anti-fibrotic therapy and regeneration of damaged lung tissue in pulmonary fibrosis. In this study, experiments were performed on male C57BL/6 mice with bleomycin-induced pulmonary fibrosis. We applied histological and immunohistochemical methods, ELISA, and assessed the expression of markers of endothelial and epithelial cells in primary cultures of CD31+ and CD326+ lung cells. Finally, we evaluated esterase activity and apoptosis of lung cells in vitro. Our data indicate that Treamid exhibits antifibrotic activity in mice with pulmonary fibrosis and has a positive effect on capillaries of the lungs. Treamid also increases the number of endothelial progenitor cells in the lungs of animals with pulmonary fibrosis. Lastly, Treamid increases esterase activity and decreases apoptosis of CD31+ lung cells in vitro. Based on these findings, we suggest that Treamid may represent a promising compound for the development of new antifibrotic agents, which are capable of stimulating regeneration of lung endothelium in IPF patients.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/93806
Identification Number/DOI	10.3390/ijms21218380
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	MDPI
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