Identification of tauopathy-associated lipid signatures in Alzheimer’s disease mouse brain using label-free chemical imaging

Meng, H., Elliott, A., Mansfield, J., Bailey, M., Frogley, M., Cinque, G., Moger, J., Stone, N., Tamagnini, F. ORCID: https://orcid.org/0000-0002-8741-5094 and Palombo, F. (2024) Identification of tauopathy-associated lipid signatures in Alzheimer’s disease mouse brain using label-free chemical imaging. Communications Biology, 7 (1). 1341. ISSN 2399-3642 doi: 10.1038/s42003-024-07034-3

Abstract/Summary

There is cumulative evidence that lipid metabolism plays a key role in the pathogenesis of various neurodegenerative disorders including Alzheimer’s disease (AD). Visualising lipid content in a non-destructive label-free manner can aid in elucidating the AD phenotypes towards a better understanding of the disease. In this study, we combined multiple optical molecular-specific methods, Fourier transform infrared (FTIR) spectroscopic imaging, synchrotron radiation-infrared (SR-IR) microscopy, Raman and stimulated Raman scattering (SRS) microscopy, and optical-photothermal infrared (O-PTIR) microscopy with multivariate data analysis, to investigate the biochemistry of brain hippocampus in situ using a mouse model of tauopathy (rTg4510). We observed a significant difference in the morphology and lipid content between transgenic (TG) and wild type (WT) samples. Immunohistochemical staining revealed some degree of microglia co-localisation with elevated lipids in the brain. These results provide new evidence of tauopathy-related dysfunction in a preclinical study at a subcellular level.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/93291
Identification Number/DOI	10.1038/s42003-024-07034-3
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	Nature Research
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