A model of the PI cycle reveals the regulating roles of lipid-binding proteins and pitfalls of using mosaic biological data

Mazet, F., Tindall, M. J., Gibbins, J. M. ORCID: https://orcid.org/0000-0002-0372-5352 and Fry, M. J. (2020) A model of the PI cycle reveals the regulating roles of lipid-binding proteins and pitfalls of using mosaic biological data. Scientific Reports, 10 (1). 13244. ISSN 2045-2322 doi: 10.1038/s41598-020-70215-7

Abstract/Summary

The phosphatidylinositol (PI) cycle is central to eukaryotic cell signaling. Its complexity, due to the number of reactions and lipid and inositol phosphate intermediates involved makes it difficult to analyze experimentally. Computational modelling approaches are seen as a way forward to elucidate complex biological regulatory mechanisms when this cannot be achieved solely through experimental approaches. Whilst mathematical modelling is well established in informing biological systems, many models are often informed by data sourced from multiple unrelated cell types (mosaic data) or from purified enzyme data. In this work, we develop a model of the PI cycle informed by experimental and omics data taken from a single cell type, namely platelets. We were able to make a number of predictions regarding the regulation of PI cycle enzymes, the importance of the number of receptors required for successful GPCR signaling and the importance of lipid- and protein-binding proteins in regulating second messenger outputs. We then consider how pathway behavior differs, when fully informed by data for HeLa cells and show that model predictions remain consistent. However, when informed by mosaic experimental data model predictions greatly vary illustrating the risks of using mosaic datasets from unrelated cell types.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/92148
Identification Number/DOI	10.1038/s41598-020-70215-7
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences Science > School of Mathematical, Physical and Computational Sciences > Department of Mathematics and Statistics
Publisher	Nature Publishing Group UK
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