Generation of high dose inhalable effervescent dispersions against Pseudomonas aeruginosa biofilms

Mohammed, A., Zurek, J., Madueke, S., Al-Kassimy, H., Yaqoob, M., Houacine, C., Ferraz, A., Kalgudi, R., Zariwala, M. G., Hawkins, N. and Al-Obaidi, H. ORCID: https://orcid.org/0000-0001-9735-0303 (2020) Generation of high dose inhalable effervescent dispersions against Pseudomonas aeruginosa biofilms. Pharmaceutical Research, 37 (8). 150. ISSN 0724-8741 doi: 10.1007/s11095-020-02878-w

Abstract/Summary

Purpose: Novel particle engineering approach was used in this study to generate high dose inhalable effervescent particles with synergistic effects against Pseudomonas aeruginosa biofilms. Methods: Spray dried co-amorphous salt of ciprofloxacin (CFX) and tartaric acid (TA) was prepared and coated with external layer of sodium bicarbonate and silica coated silver nanobeads. Design of experiments (DOE) was used to optimize physicochemical properties of particles for enhanced lung deposition. Results: Generated particles were co-amorphous CFX/TA showing that CFX lost its zwitterionic form and exhibiting distinct properties to CFX/HCl as assessed by FTIR and thermal analysis. Particles exhibited mass mean aerodynamic diameter (MMAD) of 3.3 m, emitted dose of 78% and fine particle dose of 85%. Particles were further evaluated via antimicrobial assessment of minimum inhibitory concentrations (MIC) and minimum biofilm eradication concentration (MBEC). MIC and MBEC results showed that the hybrid particles were around 3-5 times more effective when compared to CFX signifying that synergistic effect was achieved. Diffusing wave spectroscopy results showed that the silver containing particles had a disruptive effect on rheological properties as opposed to silver free particles. Conclusions: Overall, these results showed the potential to use particle engineering to generate particles that are highly disruptive of bacterial biofilms.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/91769
Item Type	Article
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Optical Spectroscopy (CAF) Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Thermal Analysis (CAF) Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Xray (CAF) Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Electron Microscopy Laboratory (CAF) Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
Publisher	Springer Verlag
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