A chiral probe for the acute phase proteins alpha-1-acid glycoprotein and alpha-1-antitrypsin based on europium luminescence

Carr, R., Di Bari, L., Lo Piano, S. ORCID: https://orcid.org/0000-0002-2625-483X, Parker, D., Peacock, R. D. and Sanderson, J. M. (2012) A chiral probe for the acute phase proteins alpha-1-acid glycoprotein and alpha-1-antitrypsin based on europium luminescence. Dalton Transactions, 41 (42). pp. 13154-13158. ISSN 1364-5447 doi: 10.1039/C2DT30143A

Abstract/Summary

Reversible and selective binding of a dynamically racemic europium(III) complex to α1-acid glycoprotein and α1-antitrypsin is characterised by a significant change in the europium total emission spectral fingerprint and the switching on of a large circularly polarised luminescence (CPL) signal from the metal centre. Observation of an induced CD into the ligand chromophore in the presence of α1-AGP allows a structure for the protein-bound complex to be postulated. A direct determination of elevated α1-AGP levels in human serum was achieved by monitoring changes in the intensity ratio of Eu emission bands.