Interactions of bile salts with a dietary fibre, methylcellulose, and impact on lipolysis

Pabois, O., Antoine-Michard, A., Zhao, X., Omar, J., Ahmed, F., Alexis, F., Harvey, R. D., Grillo, I., Gerelli, Y., Grundy, M. M.-L., Bajka, B., Wilde, P. J. and Dreiss, C. A. (2020) Interactions of bile salts with a dietary fibre, methylcellulose, and impact on lipolysis. Carbohydrate Polymers, 231. 115741. ISSN 0144-8617 doi: 10.1016/j.carbpol.2019.115741

Abstract/Summary

Methylcellulose (MC) has a demonstrated capacity to reduce fat absorption, hypothetically through bile salt (BS) activity inhibition. We investigated MC cholesterol-lowering mechanism, and compared the influence of two BS, sodium taurocholate (NaTC) and sodium taurodeoxycholate (NaTDC), which differ slightly by their architecture and exhibit contrasting functions during lipolysis. BS/MC bulk interactions were investigated by rheology, and BS behaviour at the MC/water interface studied with surface pressure and ellipsometry measurements. In vitro lipolysis studies were performed to evaluate the effect of BS on MC-stabilised emulsion droplets microstructure, with confocal microscopy, and free fatty acids release, with the pH-stat method. Our results demonstrate that BS structure dictates their interactions with MC, which, in turn, impact lipolysis. Compared to NaTC, NaTDC alters MC viscoelasticity more significantly, which may correlate with its weaker ability to promote lipolysis, and desorbs from the interface at lower concentrations, which may explain its higher propensity to destabilise emulsions.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/88064
Identification Number/DOI	10.1016/j.carbpol.2019.115741
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Animal Sciences > Animal, Dairy and Food Chain Sciences (ADFCS)- DO NOT USE
Publisher	Elsevier
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