Optimized production of Poly(γ-Glutamic acid) by bacillus sp. FBL-2 through response surface methodology using central composite design

Min, J.-H., Reddy, L. V., Charalampopoulos, D. ORCID: https://orcid.org/0000-0003-1269-8402, Kim, Y.-M. and Wee, Y.-J. (2019) Optimized production of Poly(γ-Glutamic acid) by bacillus sp. FBL-2 through response surface methodology using central composite design. Journal of Microbiology and Biotechnology, 29 (7). pp. 1061-1070. ISSN 1017-7825 doi: 10.4014/jmb.1904.04013

Abstract/Summary

In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp. FBL-2 was studied using a statistical approach. One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA. After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production. The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l. The optimized medium was validated experimentally, and γ-PGA production increased significantly from 3.59 g/l (0.33 g/l/h) to 44.04 g/l (3.67 g/l/h) when strain FBL-2 was cultivated under the optimal medium developed by the statistical approach, as compared to non-optimized medium.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/87441
Identification Number/DOI	10.4014/jmb.1904.04013
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
Publisher	Korean Society for Microbiology and Technology
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