Induction and growth of 'microspore-derived' embryos of Brassica napus ssp. oleifera

Download

Full text not archived in this repository.

Advice

Please see our End User Agreement.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

Tools

Lists

Dunwell, J. M. ORCID: https://orcid.org/0000-0003-2147-665X, Cornish, M., De Courcel, A. G. L. and Middlefell-Williams, J. E. (1983) Induction and growth of 'microspore-derived' embryos of Brassica napus ssp. oleifera. Journal of Experimental Botany, 34 (12). pp. 1768-1778. ISSN 0022-0957 doi: 10.1093/jxb/34.12.1768

Abstract/Summary

Three cultivars of spring rape (Brassica napus ssp. oleifera), Tower, Willi and Duplo, were used for a study of induction and growth of 'microspore-derived' embryos, Buds, 2.0 mm in length, containing uninucleate microspores were harvested and stored for 14 d at 4 °C in darkness. Anthers were then removed and cultured on a liquid medium based upon that of Murashige and Skoog and containing 8% sucrose, 0.5 mg l-1 naphthylacetic acid and 0-05 mg l-1 benzylaminopurine. Cultures were pre-incubated at 35 °C for 0-3 d and then incubated at 30 °C. After a total of 42 d incubation, cultures were scored for the presence of macroscopic embryos (1-2 mm in length) and for the presence of anthers containing abortive embryoids which had not developed further. The cultivars differed greatly in terms both of the frequency of anthers showing induced embryoids and of the final yield of embryos. Tower showed the highest frequency of induction (maximum 38% of cultured anthers with induced embryoids) whereas the highest yield (equivalent to 1 • 1 embryo per cultured anther) was obtained from anthers of the cv. Duplo after a 3 d treatment at 35 °C. Yields from the other cultivars were much lower and were relatively unaffected by the 35 °C treatment.

Altmetric Badge

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/86952
Identification Number/DOI	10.1093/jxb/34.12.1768
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Publisher	Oxford University Press
Download/View statistics	View download statistics for this item

Deposit Details

Date Deposited:	19 Dec 2019 10:09	Date item deposited into CentAUR
Last Modified:	23 Jun 2024 03:24	Date item last modified

References

ALTSCHULER, M., and MASCARENHAS, J.P., 1982. The synthesis of heat-shock and normal proteins at high temperatures in plants and their possible roles in survival under heat stress. In Heat shock from bacteria to man. Eds M. J. Schlesinger, M. Ashburner and A. Tissieres. Cold Spring Harbour Laboratory, U.S.A. Pp. 321-7. CHALEFF, R.S., and STOLARZ, A., 1981. Factors influencing the frequency of callus formation among cultured rice Oryza saliva anthers. Physiologia plantarum, 51, 201-6. DUNWELL, J.M, 1976. A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environmental and Experimental Botany, 16, 109-18. HANSSON, B., 1978. Temperaturchocker -ett satt att hraftigt hoja frekvensen embryoidbildningar vid antherkultur av raps (Brassica napus). Sveriges Utsadesforenings Tidskrift, 88, 141-8. KELLER, W.A., and ARMSTRONG, K.C. 1977. Embryogenesis and plant regeneration in Brassica napus anther cultures. Canadian Journal of Botany, 55, 1383-8. KELLER, W.A., and ARMSTRONG, K.C. 1978. High frequency production of microspore-derived plants from Brassica napus anther cultures. Zeitschrift fur Pflanzenzuchtung, 80, 100-8. KELLER, W.A., ARMSTRONG, K.C. and DE LA ROCHE, A.I., 1983. The production and utilization of microspore-derived haploids in Brassica crops. In Plant cell culture in crop improvement. Eds S.K. Sen and K.L. Giles. Plenum Press. New York. Pp. 169-83. KELLER, W.A., and STRINGHAM, G. R., 1978. Production and utilization of microspore-derived haploid plants. In Frontiers of plant tissue culture 1978. Ed. T. A. Thorpe. University of Calgary, Canada. Pp. 113-22. LICHTER, R., 1981. Anther culture of Brassica napus in a liquid culture medium. Zeitschrift fur Pflanzenphysiologie. 103, 229-37. LICHTER, R., 1982. Induction of haploid plants from isolated pollen of Brassica napus. Zeitschrift fur Pflanzenphysiologie. 105,427-34. LOH, C. S., and INGRAM, D. S., 1982. Production of haploid plants from anther cultures and secondary embryoids of winter oilseed rape. Brassica napus ssp. oleifera. New Phytologist, 91, 507—16. MURASHIGE, T.. and SKOOG. F.. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia plantarum, 15,473-97. RENARD, M., and DOSBA, F., 1980. Etude de l'haploidie chez le Colza (Brassica napus L. var oleifera Metzger). Annales de I 'Amelioration des Plantes, 30, 191-209. SUNDERLAND, N., COLLINS, G.B., and DUNWELL, J.M., 1974. The role of nuclear fusion in pollen embryogenesis of Datura innoxia Mill. Planta, 117, 227—41. SUNDERLAND, N., and DUNWELL. J. M., 1977. Anther and pollen culture. In Plant tissue and cell culture. Ed. H.E. Street. Blackwell, Oxford. Pp. 223-65. THOMAS, E.. and WENZEL. G.. 1975. Embryogenesis from microspores of Brassica napus. Zeitschrift fur Pflanzenzuchtung, 74, 77-81. WENZEL, G., HOFFMAN, F., and THOMAS, E., 1977. Anther culture as a breeding tool in rape. Zeitschrift fur Pflanzenzuchtung, 78, 149-55. ZEUTHEN, E., 1964. The temperature-induced division synchrony in Tetrahymena. In Synchrony in cell division and growth. Ed. E. Zeuthen. Interscience Publishers, New York. Pp. 99-158.

University Staff: Request a correction | Centaur Editors: Update this record

Search Google Scholar