Self-assembly, antimicrobial activity, and membrane interactions of arginine-capped peptide bola-amphiphiles

Edwards-Gayle, C. J. C., Castelletto, V., Hamley, I. W. ORCID: https://orcid.org/0000-0002-4549-0926, Barrett, G. ORCID: https://orcid.org/0000-0003-1509-0179, Greco, F., Hermida-Merino, D., Rambo, R. P., Seitsonen, J. and Ruokolainen, J. (2019) Self-assembly, antimicrobial activity, and membrane interactions of arginine-capped peptide bola-amphiphiles. ACS Applied Bio Materials, 2 (5). pp. 2208-2218. ISSN 2576-6422 doi: 10.1021/acsabm.9b00172

Abstract/Summary

The self-assembly and antimicrobial activity of two novel arginine-capped bola-amphiphile peptides, namely RA6R and RA9R (R, arginine; A, alanine) are investigated. RA6R does not self-assemble in water due to its high solubility, but RA9R self-assembles above a critical aggregation concentration into ordered nanofibers due to the high hydrophobicity of the A9block. The structure of the RA9R nanofibers is studied by cryogenic transmission electron microscopy (cryo-TEM) and small-angle X-ray scattering (SAXS). Circular dichroism spectroscopy shows that both RA6R and RA9R adopt coil conformations in water at low concentration, but only RA9R adopts a β-sheet conformation at high concentration. SAXS and differential scanning calorimetry are used to study RA6R and RA9R interactions with a mixed lipid membrane that models a bacterial cell wall, consisting of multilamellar 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol/1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine vesicles. Cytotoxicity studies show that RA6R is more cytocompatible than RA9R. RA6R has enhanced activity against the Gram-negative pathogen P. aeruginosa at a concentration where viability of mammalian cells is retained. RA9R has little antimicrobial activity, independently of concentration. Our results highlight the influence of the interplay between relative charge and hydrophobicity on the self-assembly, cytocompatibility, and bioactivity of peptide bola-amphiphiles.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/83426
Identification Number/DOI	10.1021/acsabm.9b00172
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	ACS Publications
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