Enzymatic hydrolysis of thermally pre‐treated chitin and antimicrobial activity of N,N'‐diacetylchitobiose

Zainal Abidin, M., Kourmentza, C., Karatzas, K. A. and Niranjan, K. ORCID: https://orcid.org/0000-0002-6525-1543 (2019) Enzymatic hydrolysis of thermally pre‐treated chitin and antimicrobial activity of N,N'‐diacetylchitobiose. Journal of Chemical Technology and Biotechnology, 94 (8). pp. 2529-2536. ISSN 1097-4660 doi: 10.1002/jctb.6043

Abstract/Summary

BACKGROUND: N,N’-diacetylchitobiose (GlcNAc2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave, has been employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from Streptomyces griseus. RESULTS: Pre-treatment of chitin with 0.05 M sodium acetate buffer (pH=6.0) at 121ºC for 60 min, followed by enzymatic hydrolysis involving 24 h incubation, was found to be the best condition for producing the GlcNAc2. The GlcNAc2 obtained was tested regarding its antimicrobial activity against Gram-negative and Gram-positive strains and showed minimum inhibitory concentrations (MIC) at 5 and 10% w/v against Escherichia coli K-12 and Listeria monocytogenes 10403S, respectively. CONCLUSION: The extent of swelling and crystallite size of chitin increased with the pre-treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/83411
Identification Number/DOI	10.1002/jctb.6043
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
Publisher	Wiley
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