Self-assembly of lipopeptides containing short peptide fragments derived from the gastrointestinal hormone PYY3–36: from micelles to amyloid fibrils

Hutchinson, J. A., Hamley, I. W. ORCID: https://orcid.org/0000-0002-4549-0926, Torras, J., Alemán, C., Seitsonen, J. and Ruokolainen, J. (2019) Self-assembly of lipopeptides containing short peptide fragments derived from the gastrointestinal hormone PYY3–36: from micelles to amyloid fibrils. Journal of Physical Chemistry B, 123 (3). pp. 614-621. ISSN 1520-6106 doi: 10.1021/acs.jpcb.8b11097

Abstract/Summary

We investigate the impact of lipidation on the self-assembly of two peptide fragments from the gastrointestinal peptide hormone PYY3–36. The lipopeptides C16IKPEAP and C16IKPEAPGE contain the first 6 and 8 amino acid residues, respectively, from the PYY3–36 peptide sequence, with a palmitoyl C16 tail attached at the N-terminus. These lipopeptides form spherical micelles in aqueous solution, above a critical micelle concentration (cmc), which is pH-dependent. Modeling of small-angle X-ray scattering data along with molecular dynamics simulations shows the formation of micelles with a hydrophobic interior and a well-hydrated exterior. The lipopeptides have a disordered conformation over the pH and temperature ranges studied. The cmc is found to be independent of temperature, pointing to athermal micellization. In contrast to the presence of hydrated micelles in solution, β-sheet amyloid fibrils form in dried samples. Thus, the nanostructure of lipidated PYY3–36 fragment peptides can be tuned by control of pH or concentration, for future applications.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/82012
Identification Number/DOI	10.1021/acs.jpcb.8b11097
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Uncontrolled Keywords	Physical and Theoretical Chemistry, Materials Chemistry, Surfaces, Coatings and Films
Publisher	American Chemical Society
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