An isotope dilution model for partitioning of phenylalanine and tyrosine uptake by the liver of lactating dairy cows

Crompton, L. A., McKnight, L. L., Reynolds, C. K. ORCID: https://orcid.org/0000-0002-4152-1190, Mills, J. A. N., Ellis, J. L., Hanigan, M. D., Dijkstra, J., Bequette, B. J., Bannink, A. and France, J. (2018) An isotope dilution model for partitioning of phenylalanine and tyrosine uptake by the liver of lactating dairy cows. Journal of Theoretical Biology, 444. pp. 100-107. ISSN 0022-5193 doi: 10.1016/j.jtbi.2017.12.016

Abstract/Summary

An isotope dilution model to describe the partitioning of phenylalanine (PHE) and tyrosine (TYR) in the bovine liver was developed. The model comprises four intracellular and six extracellular pools and various flows connecting these pools and external blood. Conservation of mass principles were applied to generate the fundamental equations describing the behaviour of the system in the steady state. The model was applied to datasets from multi-catheterised dairy cattle during a constant infusion of [1-13C] phenylalanine and [2,3,5,6-2H] tyrosine tracers. Model solutions described the extraction of PHE and TYR from the liver via the portal vein and hepatic artery. In addition, the exchange of free PHE and TYR between extracellular and intracellular pools was explained and the hydroxylation of PHE to TYR was estimated. The model was effective in providing information about the fates of PHE and TYR in the liver and could be used as part of a more complex system describing amino acid metabolism in the whole animal.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/74567
Identification Number/DOI	10.1016/j.jtbi.2017.12.016
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Animal Sciences > Animal, Dairy and Food Chain Sciences (ADFCS)- DO NOT USE
Uncontrolled Keywords	Isotope dilution, Kinetic model, Liver, Phenylalanine, Tyrosine
Publisher	Elsevier
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