Olive oil phenolics prevent oxysterol-induced proinflammatory cytokine secretion and reactive oxygen species production in human peripheral blood mononuclear cells, through modulation of p38 and JNK pathways

Serra, G., Deiana, M., Spencer, J. P. E. ORCID: https://orcid.org/0000-0003-2931-7274 and Corona, G. (2017) Olive oil phenolics prevent oxysterol-induced proinflammatory cytokine secretion and reactive oxygen species production in human peripheral blood mononuclear cells, through modulation of p38 and JNK pathways. Molecular Nutrition & Food Research, 61 (12). 1700283. ISSN 1613-4125 doi: 10.1002/mnfr.201700283

Abstract/Summary

The aim of the present study was to investigate the ability of extra virgin olive oil (EVOO) polyphenols to counteract the proinflammatory effects induced by dietary and endogenous oxysterols in ex vivo immune cells. Peripheral blood mononuclear cells (PBMCs), separated from the whole blood of healthy donors, were utilized and were stimulated with an oxysterols mixture, in the presence of physiologically relevant concentrations of the EVOO polyphenols, hydroxytyrosol, tyrosol, and homovanillic alcohol. Oxysterols significantly increased the production of proinflammatory cytokines, interleukin-1β, regulated on activation, normal T-cell expressed and secreted and macrophage migration inhibitory factor in ex vivo cultured PBMCs. Increased levels of reactive oxygen species (ROS) were also detected along with increased phosphorylation of the p38 and JNK. All phenolic compounds significantly reduced cytokine secretion induced by the oxysterols and inhibited ROS production and mitogen activated protein kinase phosphorylation. These results suggest that extra virgin olive oil polyphenols modulate the immune response induced by dietary and endogenous cholesterol oxidation products in human immune cells and may hold benefit in controlling chronic immune and/or inflammatory processes.

Additional Information	** Embargo End Date: 26-10-2017 ** From Crossref via Jisc Publications Router. ** Licence for vor version of this article starting on 26-10-2017: http://creativecommons.org/licenses/by/4.0/
Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/73446
Identification Number/DOI	10.1002/mnfr.201700283
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
Uncontrolled Keywords	Biotechnology, Food Science
Additional Information	** Embargo End Date: 26-10-2017 ** From Crossref via Jisc Publications Router. ** Licence for vor version of this article starting on 26-10-2017: http://creativecommons.org/licenses/by/4.0/
Publisher	Wiley
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