Inhibition of ICE-family cysteine proteases rescues murine lymphocytes from lipoxygenase inhibitor-induced apoptosis

Mirzoeva, O., Yaqoob, P. ORCID: https://orcid.org/0000-0002-6716-7599, Knox, K. and Calder, P. (1996) Inhibition of ICE-family cysteine proteases rescues murine lymphocytes from lipoxygenase inhibitor-induced apoptosis. FEBS Letters, 396. pp. 266-270. ISSN 0014-5793

Abstract/Summary

Two lipophilic derivatives of caffein acid which inhibit lipoxygenase, caffeic acid phenethyl ester (CAPE) and N,N'- dicyclohexyl-O-(3,4-dihydroxycinnamoyl)-isourea (DCHCU), reduced the proliferative response of murine splenocytes to concanavalin A in vitro. Both CAPE and DCHCU induced apoptosis in murine thymocyte cultures as verified by flow cytometry and by visualisation of DNA with acridine orange staining. CAPE-induced apoptosis was inhibited by z-VAD-fmk, an inhibitor of the interleukin-l[~-converting enzyme family of cysteine proteases. We suggest that the lipoxygenase pathway of arachidonic acid metabolism plays a role in regulating lympho- cyte responses such as proliferation and apoptosis.