Microsatellite markers for hoop-petticoat daffodils (Narcissus sect. Bulbocodii; Amaryllidaceae)

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Konyves, K., David, J. C. and Culham, A. ORCID: https://orcid.org/0000-0002-7440-0133 (2016) Microsatellite markers for hoop-petticoat daffodils (Narcissus sect. Bulbocodii; Amaryllidaceae). Applications in Plant Sciences, 4 (4). 1500127. ISSN 2168-0450 doi: 10.3732/apps.1500127

Abstract/Summary

• Premise of the study: Microsatellite markers were developed using hoop-petticoat daffodils ( Narcissus sect. Bulbocodii ; Amaryllidaceae) to aid in the taxonomic revision of the section, and further to evaluate their broad applicability for daffodil cultivar identification. • Methods and Results: Three hundred fifty-one primer pairs were developed using a commercial service. Nineteen polymorphic and repeatable markers were developed by screening 67 of these primer pairs. Of these, 11 chosen markers were used to screen 317 samples; the number of alleles per locus ranged from four to 21, and the observed heterozygosity ranged from 0.101 to 0.297. There were null genotypes in some samples for six of the markers. All the microsatellites were transferable to other Narcissus sections. • Conclusions: The results indicate that these new markers have sufficient potential variation to be used for taxonomic revision of the genus and to distinguish many commercial daffodil cultivars.

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Additional Information	Funded by the Royal Horticultural Society
Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/62397
Identification Number/DOI	10.3732/apps.1500127
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Ecology and Evolutionary Biology Central Services > Academic and Governance Services > University Museums and Special Collections
Uncontrolled Keywords	microsatellite, taxonomy, DNA marker, Daffodil, Narcissus, Amaryllidaceae, daffodil cultivars, horticultural taxonomy; microsatellite markers; Narcissus section Bulbocodii, polyploidy
Additional Information	Funded by the Royal Horticultural Society
Publisher	Botanical Society of America
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Date Deposited:	22 Apr 2016 13:44	Date item deposited into CentAUR
Last Modified:	23 Jun 2024 04:56	Date item last modified

References

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