Design, implementation and interpretation of in vitro batchculture experiments to assess enteric methane mitigation in ruminants—a review

Yáñez-Ruiza, D. R., Bannink, A., Dijkstra, J., Kebreab, E., Morgavi, D.P., O’Kiely, P., Reynolds, C. K. ORCID: https://orcid.org/0000-0002-4152-1190, Schwarm, A., Shingfield, K. J., Yu, Z. and Hristov, A. N. (2016) Design, implementation and interpretation of in vitro batchculture experiments to assess enteric methane mitigation in ruminants—a review. Animal Feed Science and Technology, 216. pp. 1-18. ISSN 0377-8401 doi: 10.1016/j.anifeedsci.2016.03.016

Abstract/Summary

In vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritivevalue of feeds for ruminants and in the last decade to assess the effect of different nutritionalstrategies on methane (CH4) production. However, many technical factors may influencethe results obtained. The present review has been prepared by the ‘Global Network’ FACCE-JPI international research consortium to provide a critical evaluation of the main factorsthat need to be considered when designing, conducting and interpreting IVFT experimentsthat investigate nutritional strategies to mitigate CH4emission from ruminants. Given theincreasing and wide-scale use of IVFT, there is a need to critically review reports in the lit-erature and establish what criteria are essential to the establishment and implementationof in vitro techniques. Key aspects considered include: i) donor animal species and numberof animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluidas inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures andCH4measurements, vi) headspace gas composition and vii) comparability of in vitro andin vivo measurements. Based on an evaluation of experimental evidence, a set of techni-cal recommendations are presented to harmonize IVFT for feed evaluation, assessment ofrumen function and CH4production.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/62104
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Animal Sciences > Animal, Dairy and Food Chain Sciences (ADFCS)- DO NOT USE
Uncontrolled Keywords	Feed evaluation; In vitro gas production; Methane; Rumen; Mitigation; Microbial inoculum
Publisher	Elsevier
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