Distribution analysis of the putative cancer marker S100A4 across invasive squamous cell carcinoma penile tissue

Flatley, B., Quaye, C., Johnson, E., Freeman, A., Muneer, A., Minhas, S., Paterson, J. C., Musa, F., Malone, P. and Cramer, R. ORCID: https://orcid.org/0000-0002-8037-2511 (2015) Distribution analysis of the putative cancer marker S100A4 across invasive squamous cell carcinoma penile tissue. EuPA Open Proteomics, 7. pp. 1-10. ISSN 2212-9685 doi: 10.1016/j.euprot.2015.02.001

Abstract/Summary

MS-based proteomic methods were utilised for the first time in the discovery of novel penile cancer biomarkers. MALDI MS imaging was used to obtain the in situ biomolecular MS profile of squamous cell carcinoma of the penis which was then compared to benign epithelial MS profiles. Spectra from cancerous and benign tissue areas were examined to identify MS peaks that best distinguished normal epithelial cells from invasive squamous epithelial cells, providing crucial evidence to suggest S100A4 to be differentially expressed. Verification by immunohistochemistry resulted in positive staining for S100A4 in a sub-population of invasive but not benign epithelial cells.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/58176
Identification Number/DOI	10.1016/j.euprot.2015.02.001
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	Elsevier
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