A novel interaction between FlnA and Syk regulates platelet ITAM-mediated receptor signaling and function

Falet, H., Pollitt, A. Y. ORCID: https://orcid.org/0000-0001-8706-5154, Begonja, A. J., Weber, S. E., Duerschmied, D., Wagner, D. D., Watson, S. P. and Hartwig, J. H. (2010) A novel interaction between FlnA and Syk regulates platelet ITAM-mediated receptor signaling and function. The Journal of Experimental Medicine, 207 (9). pp. 1967-1979. ISSN 1540-9538 doi: 10.1084/jem.20100222

Abstract/Summary

Filamin A (FlnA) cross-links actin filaments and connects the Von Willebrand factor receptor GPIb-IX-V to the underlying cytoskeleton in platelets. Because FlnA deficiency is embryonic lethal, mice lacking FlnA in platelets were generated by breeding FlnA(loxP/loxP) females with GATA1-Cre males. FlnA(loxP/y) GATA1-Cre males have a macrothrombocytopenia and increased tail bleeding times. FlnA-null platelets have decreased expression and altered surface distribution of GPIbalpha because they lack the normal cytoskeletal linkage of GPIbalpha to underlying actin filaments. This results in approximately 70% less platelet coverage on collagen-coated surfaces at shear rates of 1,500/s, compared with wild-type platelets. Unexpectedly, however, immunoreceptor tyrosine-based activation motif (ITAM)- and ITAM-like-mediated signals are severely compromised in FlnA-null platelets. FlnA-null platelets fail to spread and have decreased alpha-granule secretion, integrin alphaIIbbeta3 activation, and protein tyrosine phosphorylation, particularly that of the protein tyrosine kinase Syk and phospholipase C-gamma2, in response to stimulation through the collagen receptor GPVI and the C-type lectin-like receptor 2. This signaling defect was traced to the loss of a novel FlnA-Syk interaction, as Syk binds to FlnA at immunoglobulin-like repeat 5. Our findings reveal that the interaction between FlnA and Syk regulates ITAM- and ITAM-like-containing receptor signaling and platelet function.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/44796
Identification Number/DOI	10.1084/jem.20100222
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	Rockefeller University Press
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