G6f-like is an ITAM-containing collagen receptor in thrombocytes

Hughes, C. E. ORCID: https://orcid.org/0000-0002-9790-5820, Radhakrishnan, U. P., Lordkipanidzé, M., Egginton, S., Dijkstra, J. M., Jagadeeswaran, P. and Watson, S. P. (2012) G6f-like is an ITAM-containing collagen receptor in thrombocytes. PLoS ONE, 7 (12). e52622. ISSN 1932-6203 doi: 10.1371/journal.pone.0052622

Abstract/Summary

Collagen activates mammalian platelets through a complex of the immunoglobulin (Ig) receptor GPVI and the Fc receptor γ-chain, which has an immunoreceptor tyrosine-based activation motif (ITAM). Cross-linking of GPVI mediates activation through the sequential activation of Src and Syk family kinases and activation of PLCγ2. Nucleated thrombocytes in fish are activated by collagen but lack an ortholog of GPVI. In this study we show that collagen activates trout thrombocytes in whole blood and under flow conditions through a Src kinase driven pathway. We identify the Ig receptor G6f-like as a collagen receptor and demonstrate in a cell line assay that it signals through its cytoplasmic ITAM. Using a morpholino for in vivo knock-down of G6f-like levels in zebrafish, we observed a marked delay or absence of occlusion of the venous and arterial systems in response to laser injury. Thus, G6f-like is a physiologically relevant collagen receptor in fish thrombocytes which signals through the same ITAM-based signalling pathway as mammalian GPVI, providing a novel example of convergent evolution.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/44571
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	Public Library of Science
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