Syk and Src family kinases regulate C-type lectin receptor 2 (CLEC-2)-mediated clustering of podoplanin and platelet adhesion to lymphatic endothelial cells

Pollitt, A. Y. ORCID: https://orcid.org/0000-0001-8706-5154, Poulter, N. S., Gitz, E., Navarro-Nuñez, L., Wang, Y.-J., Hughes, C. E. ORCID: https://orcid.org/0000-0002-9790-5820, Thomas, S. G., Nieswandt, B., Douglas, M. R., Owen, D. M., Jackson, D. G., Dustin, M. L. and Watson, S. P. (2014) Syk and Src family kinases regulate C-type lectin receptor 2 (CLEC-2)-mediated clustering of podoplanin and platelet adhesion to lymphatic endothelial cells. The Journal of Biological Chemistry, 289 (52). pp. 35695-35710. ISSN 1083-351X doi: 10.1074/jbc.M114.584284

Abstract/Summary

The interaction of C-type lectin receptor 2 (CLEC-2) on platelets with Podoplanin on lymphatic endothelial cells initiates platelet signaling events that are necessary for prevention of blood-lymph mixing during development. In the present study, we show that CLEC-2 signaling via Src family and Syk tyrosine kinases promotes platelet adhesion to primary mouse lymphatic endothelial cells at low shear. Using supported lipid bilayers containing mobile Podoplanin, we further show that activation of Src and Syk in platelets promotes clustering of CLEC-2 and Podoplanin. Clusters of CLEC-2-bound Podoplanin migrate rapidly to the center of the platelet to form a single structure. Fluorescence lifetime imaging demonstrates that molecules within these clusters are within 10 nm of one another and that the clusters are disrupted by inhibition of Src and Syk family kinases. CLEC-2 clusters are also seen in platelets adhered to immobilized Podoplanin using direct stochastic optical reconstruction microscopy. These findings provide mechanistic insight by which CLEC-2 signaling promotes adhesion to Podoplanin and regulation of Podoplanin signaling, thereby contributing to lymphatic vasculature development.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/44566
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	American Society for Biochemistry and Molecular Biology
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