The APOB insertion/deletion polymorphism (rs17240441) influences postprandial lipaemia in healthy adults

Vimaleswaran, K. S. ORCID: https://orcid.org/0000-0002-8485-8930, Minihane, A. M., Li, Y., Gill, R., Lovegrove, J. A. ORCID: https://orcid.org/0000-0001-7633-9455, Williams, C. M. and Jackson, K. G. ORCID: https://orcid.org/0000-0002-0070-3203 (2015) The APOB insertion/deletion polymorphism (rs17240441) influences postprandial lipaemia in healthy adults. Nutrition & Metabolism, 12 (1). 7. ISSN 1743-7075 doi: 10.1186/s12986-015-0002-9

Abstract/Summary

BACKGROUND: Apolipoprotein (apo)B is the structural apoprotein of intestinally- and liver- derived lipoproteins and plays an important role in the transport of triacylglycerol (TAG) and cholesterol. Previous studies have examined the association between the APOB insertion/deletion (ins/del) polymorphism (rs17240441) and postprandial lipaemia in response to a single meal; however the findings have been inconsistent with studies often underpowered to detect genotype-lipaemia associations, focused mainly on men, or with limited postprandial characterisation of participants. In the present study, using a novel sequential test meal protocol which more closely mimics habitual eating patterns, we investigated the impact of APOB ins/del polymorphism on postprandial TAG, non-esterified fatty acids, glucose and insulin levels in healthy adults. FINDINGS: Healthy participants (n = 147) consumed a standard test breakfast (0 min; 49 g fat) and lunch (330 min; 29 g fat), with blood samples collected before (fasting) and on 11 subsequent occasions until 480 min after the test breakfast. The ins/ins homozygotes had higher fasting total cholesterol, LDL-cholesterol, TAG, insulin and HOMA-IR and lower HDL-cholesterol than del/del homozygotes (P < 0.017). A higher area under the time response curve (AUC) was evident for the postprandial TAG (P < 0.001) and insulin (P = 0.032) responses in the ins/ins homozygotes relative to the del/del homozygotes, where the genotype explained 35% and 7% of the variation in the TAG and insulin AUCs, respectively. CONCLUSIONS: In summary, our findings indicate that the APOB ins/del polymorphism is likely to be an important genetic determinant of the large inter-individual variability in the postprandial TAG and insulin responses to dietary fat intake.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/39774
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
Publisher	BioMed Central Ltd
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