Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots

Sporbert, A., Cseresnyes, Z., Heidbreder, M., Domaing, P., Hauser, S., Kaltschmidt, B., Kaltschmidt, C., Heilemann, M. and Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X (2013) Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots. PLoS ONE, 8 (5). e64023. ISSN 1932-6203 doi: 10.1371/journal.pone.0064023

Abstract/Summary

This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/39517
Identification Number/DOI	10.1371/journal.pone.0064023
Refereed	Yes
Divisions	No Reading authors. Back catalogue items Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	Public Library of Science
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