Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots

Heidbreder, M., Endesfelder, U., van de Linde, S., Hennig, S., Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X, Kaltschmidt, B., Kaltschmidt, C. and Heilemann, M. (2010) Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. Biochimica Et Biophysica Acta-Molecular Cell Research, 1803 (10). pp. 1224-1229. ISSN 0167-4889 doi: 10.1016/j.bbamcr.2010.06.004

Abstract/Summary

We introduce semiconductor quantum dot-based fluorescence imaging with approximately 2-fold increased optical resolution in three dimensions as a method that allows both studying cellular structures and spatial organization of biomolecules in membranes and subcellular organelles. Target biomolecules are labelled with quantum dots via immunocytochemistry. The resolution enhancement is achieved by three-photon absorption of quantum dots and subsequent fluorescence emission from a higher-order excitonic state. Different from conventional multiphoton microscopy, this approach can be realized on any confocal microscope without the need for pulsed excitation light. We demonstrate quantum dot triexciton imaging (QDTI) of the microtubule network of U373 cells, 3D imaging of TNF receptor 2 on the plasma membrane of HeLa cells, and multicolor 3D imaging of mitochondrial cytochrome c oxidase and actin in COS-7 cells.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/39505
Identification Number/DOI	10.1016/j.bbamcr.2010.06.004
Refereed	Yes
Divisions	No Reading authors. Back catalogue items Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	Elsevier
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