Morphological characterization of periodontium-derived human stem cells

Arnold, W. H., Becher, S., Dannan, A., Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X, Dittmar, T., Jacob, M., Mannherz, H. G., Dittmar, T., Kaltschmidt, B., Kaltschmidt, C. and Grimm, W.-D. (2010) Morphological characterization of periodontium-derived human stem cells. Annals of Anatomy - Anatomischer Anzeiger, 192 (4). pp. 215-219. ISSN 1618-0402 doi: 10.1016/j.aanat.2010.05.004

Abstract/Summary

The aim of this study has been to characterize adult human somatic periodontium-derived stem cells (PDSCS) isolated from human periodontium and to follow their differentiation after cell culture. PDSCS were isolated from human periodontal tissue and cultured as spheres in serum-free medium. After 10 days the primary spheres were dissociated and the secondary spheres sub-cultured for another 1-2 weeks. Cells from different time points were analyzed, and immunohistochemical and electron microscopic investigations carried out. Histological analysis showed differentiation of spheres deriving from the PDSCS with central production of extracellular matrix beginning 3 days after sub-culturing. Isolated PDSCS developed pseudopodia which contained actin. Tubulin was found in the central portion of the cells. Pseudopodia between different cells anastomosed, indicating intercellular transport. Immunostaining for osteopontin demonstrated a positive reaction in primary spheres and within extracellular matrix vesicles after sub-culturing. In cell culture under serum-free conditions human PDSCS form spheres which are capable of producing extracellular matrix. Further investigations have do be carried out to investigate the capability of these cells to differentiate into osteogenic progenitor cells.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/39504
Identification Number/DOI	10.1016/j.aanat.2010.05.004
Refereed	Yes
Divisions	No Reading authors. Back catalogue items Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	Science Direct
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