Identification and quantification of glucosinolate and flavonol compounds in rocket salad (Eruca sativa, Eruca vesicaria and Diplotaxis tenuifolia) by LC–MS: highlighting the potential for improving nutritional value of rocket crops

Bell, L. ORCID: https://orcid.org/0000-0003-2895-2030, Oruna-Concha, M. J. ORCID: https://orcid.org/0000-0001-7916-1592 and Wagstaff, C. ORCID: https://orcid.org/0000-0001-9400-8641 (2015) Identification and quantification of glucosinolate and flavonol compounds in rocket salad (Eruca sativa, Eruca vesicaria and Diplotaxis tenuifolia) by LC–MS: highlighting the potential for improving nutritional value of rocket crops. Food Chemistry, 172. pp. 852-861. ISSN 0308-8146 doi: 10.1016/j.foodchem.2014.09.116

Abstract/Summary

Liquid Chromatography Mass Spectrometry (LC-MS) was used to obtain glucosinolate and flavonol content for 35 rocket accessions and commercial varieties. 13 glucosinolates and 11 flavonol compounds were identified. Semi-quantitative methods were used to estimate concentrations of both groups of compounds. Minor glucosinolate composition was found to be different between accessions; concentrations varied significantly. Flavonols showed differentiation between genera, with Diplotaxis accumulating quercetin glucosides and Eruca accumulating kaempferol glucosides. Several compounds were detected in each genus that have only previously been reported in the other. We highlight how knowledge of phytochemical content and concentration can be used to breed new, nutritionally superior varieties. We also demonstrate the effects of controlled environment conditions on the accumulations of glucosinolates and flavonols and explore the reasons for differences with previous studies. We stress the importance of consistent experimental design between research groups to effectively compare and contrast results.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/37943
Identification Number/DOI	10.1016/j.foodchem.2014.09.116
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Centre for Food Security Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
Publisher	Elsevier
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