An eight-parent multiparent advanced generation inter-cross population for winter-sown wheat: creation, properties, and validation

Mackay, I. J., Bansept-Basler, P., Barber, T., Bentley, A. R., Cockram, J., Gosman, N., Greenland, A. J., Horsnell, R., Howells, R., O'Sullivan, D. M. ORCID: https://orcid.org/0000-0003-4889-056X, Rose, G. A. and Howell, P. J. (2014) An eight-parent multiparent advanced generation inter-cross population for winter-sown wheat: creation, properties, and validation. G3, 4 (9). pp. 1603-1610. ISSN 2160-1836 doi: 10.1534/g3.114.012963

Abstract/Summary

MAGIC populations represent one of a new generation of crop genetic mapping resources combining high genetic recombination and diversity. We describe the creation and validation of an eight-parent MAGIC population consisting of 1091 F7 lines of winter-sown wheat (Triticum aestivum L.). Analyses based on genotypes from a 90,000-single nucleotide polymorphism (SNP) array find the population to be well-suited as a platform for fine-mapping quantitative trait loci (QTL) and gene isolation. Patterns of linkage disequilibrium (LD) show the population to be highly recombined; genetic marker diversity among the founders was 74% of that captured in a larger set of 64 wheat varieties, and 54% of SNPs segregating among the 64 lines also segregated among the eight founder lines. In contrast, a commonly used reference bi-parental population had only 54% of the diversity of the 64 varieties with 27% of SNPs segregating. We demonstrate the potential of this MAGIC resource by identifying a highly diagnostic marker for the morphological character "awn presence/absence" and independently validate it in an association-mapping panel. These analyses show this large, diverse, and highly recombined MAGIC population to be a powerful resource for the genetic dissection of target traits in wheat, and it is well-placed to efficiently exploit ongoing advances in phenomics and genomics. Genetic marker and trait data, together with instructions for access to seed, are available at http://www.niab.com/MAGIC/.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/37795
Identification Number/DOI	10.1534/g3.114.012963
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Centre for Food Security Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Uncontrolled Keywords	quantitative genetics genome-wide association scan single nucleotide polymorphism QTL mapping recombinant inbred line (RIL) high-density genotyping Multiparent Advanced Generation Inter-Cross (MAGIC) multiparental populations MPP
Publisher	Genetics Society of America
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