Drug permeability in 16HBE14o- airway cell layers correlates with absorption from the isolated perfused rat lung

Manford, F., Tronde, A., Jeppsson, A.-B., Patel, N. ORCID: https://orcid.org/0000-0002-8600-0663, Johansson, F. and Forbes, B. (2005) Drug permeability in 16HBE14o- airway cell layers correlates with absorption from the isolated perfused rat lung. European Journal of Pharmaceutical Sciences, 26 (5). pp. 414-20. ISSN 0928-0987 doi: 10.1016/j.ejps.2005.07.010

Abstract/Summary

The permeability of the lung is critical in determining the disposition of inhaled drugs and the respiratory epithelium provides the main physical barrier to drug absorption. The 16HBE14o- human bronchial epithelial cell line has been developed recently as a model of the airway epithelium. In this study, the transport of 10 low molecular weight compounds was measured in the 16HBE14o- cell layers, with apical to basolateral (absorptive) apparent permeability coefficients (P(app)) ranging from 0.4 x 10(-6)cms(-1) for Tyr-D-Arg-Phe-Phe-NH(2) to 25.2x10(-6)cms(-1) for metoprolol. Permeability in 16HBE14o- cells was found to correlate with previously reported P(app) in Caco-2 cells and absorption rates in the isolated perfused rat lung (k(a,lung)) and the rat lung in vivo (k(a,in vivo)). Log linear relationships were established between P(app) in 16HBE14o- cells and P(app) in Caco-2 cells (r(2)=0.82), k(a,lung) (r(2)=0.78) and k(a,in vivo) (r(2)=0.68). The findings suggest that permeability in 16HBE14o- cells may be useful to predict the permeability of compounds in the lung, although no advantage of using the organ-specific cell line 16HBE14o- compared to Caco-2 cells was found in this study.