Purification of coronavirus virions for Cryo-EM and proteomic analysis

Dent, S. and Neuman, B. (2014) Purification of coronavirus virions for Cryo-EM and proteomic analysis. Methods in Molecular Biology, 1282. pp. 99-108. ISSN 1064-3745 doi: 10.1007/978-1-4939-2438-7_10

Abstract/Summary

Purification of intact enveloped virus particles can be useful as a first step in understanding the structure and function of both viral and host proteins that are incorporated into the virion. Purified preparations of virions can be used to address these questions using techniques such as mass spectrometry proteomics. Recent studies on the proteome of coronavirus virions have shown that in addition to the structural proteins, accessory and non-structural virus proteins and a wide variety of host cell proteins associate with virus particles. To further study the presence of virion proteins, high quality sample preparation is crucial to ensure reproducible analysis by the wide variety of methods available for proteomic analysis.