The trafficking pathway of a wheat storage protein in transgenic rice endosperm

Oszvald, M., Tamas, L., Shewry, P. R. and Tosi, P. ORCID: https://orcid.org/0000-0003-4171-6120 (2014) The trafficking pathway of a wheat storage protein in transgenic rice endosperm. Annals of Botany, 113 (5). pp. 807-815. ISSN 0305-7364 doi: 10.1093/aob/mcu008

Abstract/Summary

Background and Aims The trafficking of proteins in the endoplasmic reticulum (ER) of plant cells is a topic of considerable interest since this organelle serves as an entry point for proteins destined for other organelles, as well as for the ER itself. In the current work, transgenic rice was used to study the pattern and pathway of deposition of the wheat high molecular weight (HMW) glutenin sub-unit (GS) 1Dx5 within the rice endosperm using specific antibodies to determine whether it is deposited in the same or different protein bodies from the rice storage proteins, and whether it is located in the same or separate phases within these. Methods The protein distribution and the expression pattern of HMW sub-unit 1Dx5 in transgenic rice endosperm at different stages of development were determined using light and electron microscopy after labelling with antibodies. Key results The use of HMW-GS-specific antibodies showed that sub-unit 1Dx5 was expressed mainly in the sub-aleurone cells of the endosperm and that it was deposited in both types of protein body present in the rice endosperm: derived from the ER and containing prolamins, and derived from the vacuole and containing glutelins. In addition, new types of protein bodies were also formed within the endosperm cells. Conclusions The results suggest that the HMW 1Dx5 protein could be trafficked by either the ER or vacuolar pathway, possibly depending on the stage of development, and that its accumulation in the rice endosperm could compromise the structural integrity of protein bodies and their segregation into two distinct populations in the mature endosperm.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/36822
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Uncontrolled Keywords	HMW-GS immunolocalization protein trafficking protein bodies cereal endosperm transgenic rice Oryza sativa wheat Triticum aestivum
Download/View statistics	View download statistics for this item