A comparison of thiolated and disulfide‐crosslinked polyethylenimine for nonviral gene delivery

Aravindan, L., Bicknell, K.A. ORCID: https://orcid.org/0000-0002-5888-1424, Brooks, G., Khutoryanskiy, V.V. ORCID: https://orcid.org/0000-0002-7221-2630 and Williams, A.C. ORCID: https://orcid.org/0000-0003-3654-7916 (2013) A comparison of thiolated and disulfide‐crosslinked polyethylenimine for nonviral gene delivery. Macromolecular Bioscience, 13 (9). pp. 1163-1173. ISSN 1616-5187 doi: 10.1002/mabi.201300103

Abstract/Summary

Branched polyethylenimine (25 kDa) is thiolated and compared with redox-sensitive crosslinked derivatives. Both polymers thiol contents are assessed; the thiolated polymers have 390–2300 mmol SH groups/mol, whereas the crosslinked polymers have lower thiol contents. Cytotoxicity assays show that both modified polymers give lower hemolysis than unmodified PEI. Increased thiol content increases gene transfer efficiency but also elevates cytotoxicity. Crosslinking improves plasmid DNA condensation and enhances transfection efficiency, but extensive crosslinking overstabilizes the polyplexes and decreases transfection, emphasizing the need to balance polyplex stabilization and unpacking. Thus, at low levels of crosslinking, 25 kDa PEI can be an efficient redox-sensitive carrier system.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/34304
Item Type	Article
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Thermal Analysis (CAF) Life Sciences > School of Biological Sciences > Biomedical Sciences Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
Publisher	Wiley
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