Integration of retrotransposons-based markers in a linkage map of barley

Rodriguez, M., O'Sullivan, D. M. ORCID: https://orcid.org/0000-0003-4889-056X, Donini, P., Papa, R., Chiapparino, E., Leigh, F. and Attene, G. (2006) Integration of retrotransposons-based markers in a linkage map of barley. Molecular Breeding, 17 (2). pp. 173-184. ISSN 1572-9788 doi: 10.1007/s11032-005-4885-4

Abstract/Summary

A deeper understanding of random markers is important if they are to be employed for a range of objectives. The sequence specific amplified polymorphism (S-SAP) technique is a powerful genetic analysis tool which exploits the high copy number of retrotransposon long terminal repeats (LTRs) in the plant genome. The distribution and inheritance of S-SAP bands in the barley genome was studied using the Steptoe × Morex (S × M) double haploid (DH) population. Six S-SAP primer combinations generated 98 polymorphic bands, and map positions were assigned to all but one band. Eight putative co-dominant loci were detected, representing 16 of the mapped markers. Thus at least 81 of the mapped S-SAP loci were dominant. The markers were distributed along all of the seven chromosomes and a tendency to cluster was observed. The distribution of S-SAP markers over the barley genome concurred with the knowledge of the high copy number of retrotransposons in plants. This experiment has demonstrated the potential for the S-SAP technique to be applied in a range of analyses such as genetic fingerprinting, marker assisted breeding, biodiversity assessment and phylogenetic analyses.