The integration of proteomics and systems approaches to map regulatory mechanisms underpinning platelet function.

Wright, B., Stanley, R. G., Kaiser, W. J. and Gibbins, J. M. ORCID: https://orcid.org/0000-0002-0372-5352 (2013) The integration of proteomics and systems approaches to map regulatory mechanisms underpinning platelet function. Proteomics, Clinical Applications, 7 (1-2). pp. 144-154. ISSN 1862-8354 doi: 10.1002/prca.201200095

Abstract/Summary

Platelets in the circulation are triggered by vascular damage to activate, aggregate and form a thrombus that prevents excessive blood loss. Platelet activation is stringently regulated by intracellular signalling cascades, which when activated inappropriately lead to myocardial infarction and stroke. Strategies to address platelet dysfunction have included proteomics approaches which have lead to the discovery of a number of novel regulatory proteins of potential therapeutic value. Global analysis of platelet proteomes may enhance the outcome of these studies by arranging this information in a contextual manner that recapitulates established signalling complexes and predicts novel regulatory processes. Platelet signalling networks have already begun to be exploited with interrogation of protein datasets using in silico methodologies that locate functionally feasible protein clusters for subsequent biochemical validation. Characterization of these biological systems through analysis of spatial and temporal organization of component proteins is developing alongside advances in the proteomics field. This focused review highlights advances in platelet proteomics data mining approaches that complement the emerging systems biology field. We have also highlighted nucleated cell types as key examples that can inform platelet research. Therapeutic translation of these modern approaches to understanding platelet regulatory mechanisms will enable the development of novel anti-thrombotic strategies.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/30828
Item Type	Article
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR) Life Sciences > School of Biological Sciences > Biomedical Sciences
Uncontrolled Keywords	platelets, proteomics,in silico
Publisher	Wiley
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