Bacillus subtilis spores competitively exclude Escherichia coli O78:K80 in poultry

La Ragione, R.M., Casula, G., Cutting, S.M. and Woodward, M.J. (2001) Bacillus subtilis spores competitively exclude Escherichia coli O78:K80 in poultry. Veterinary Microbiology, 79 (2). pp. 133-142. ISSN 0378-1135 doi: 10.1016/s0378-1135(00)00350-3

Abstract/Summary

Newly hatched specific pathogen-free chicks were dosed with a suspension of Bacillus subtilis spores prior to challenge with Escherichia coli O78:K80, a known virulent strain associated with avian colibacillosis. 24 h later. A single oral inoculum of 2.5 x 10(8) spores was sufficient to suppress all aspects of E. coli O78:K80 infection. Colonisation of deep organs was reduced by a factor of over 2 log(10) whilst colonisation of the intestine, as measured by direct caecal count, was reduced over 3 log(10). Shedding of E. coli O78:K80 was measured by semi-quantitative cloacal swabbing and was reduced significantly for the: duration of the experiment, 35 days. B, subtilis persisted in the intestine although with decreasing numbers over the same period. Challenge with the same dose 5 days after pre-dosing with spores overcame any suppressive effect of the spores. Crown Copyright (C) 2001 Published by Elsevier Science B.V. All rights reserved.