Measurement of intrinsic properties of amyloid fibrils by the peak force QNM method

Adamcik, J., Lara, C., Usov, I., Jeong, J. S., Ruggeri, F. S., Dietler, G., Lashuel, H. A., Hamley, I. ORCID: https://orcid.org/0000-0002-4549-0926 and Mezzenga, R. (2012) Measurement of intrinsic properties of amyloid fibrils by the peak force QNM method. Nanoscale, 4 (15). pp. 4426-4429. ISSN 2040-3364 doi: 10.1039/c2nr30768e

Abstract/Summary

We report the investigation of the mechanical properties of different types of amyloid fibrils by the peak force quantitative nanomechanical (PF-QNM) technique. We demonstrate that this technique correctly measures the Young’s modulus independent of the polymorphic state and the cross-sectional structural details of the fibrils, and we show that values for amyloid fibrils assembled from heptapeptides, a-synuclein, Ab(1–42), insulin, b-lactoglobulin,lysozyme, ovalbumin, Tau protein and bovine serum albumin all fall in the range of 2–4 GPa.