Interaction of enterohemorrhagic Escherichia coli O157 : H7 with mouse intestinal mucosa

Girard, F., Frankel, G., Phillips, A. D., Cooley, W., Weyer, U., Dugdale, A. H. A., Woodward, M. J. and La Ragione, R. M. (2008) Interaction of enterohemorrhagic Escherichia coli O157 : H7 with mouse intestinal mucosa. FEMS Microbiology Letters, 283 (2). pp. 196-202. ISSN 0378-1097 doi: 10.1111/j.1574-6968.2008.01166.x

Abstract/Summary

In this study, we used mouse ileal loops to investigate the interaction of enterohemorrhagic Escherichia coli (EHEC) O157:H7 with the mouse intestinal mucosa. With a dose of 10(9) and 3 h incubation, EHEC O157 was detected in the lumen and to a lesser extent associated with the epithelium. Typical attaching and effacing (A/E) lesions were seen, albeit infrequently. While the effector protein Tir was essential for A/E lesion formation, the bacterial type III secretion system adaptor protein TccP was dispensable. These results suggest that A/E lesions on mouse intestinal mucosa can be formed independently of robust actin polymerization.