Tissue engineering a fetal membrane

Mi, S., David, A. L., Chowdhury, B., Jones, R. R., Hamley, I. ORCID: https://orcid.org/0000-0002-4549-0926, Squires, A. and Connon, C. J. (2012) Tissue engineering a fetal membrane. Tissue Engineering Part A, 18 (3-4). pp. 373-381. ISSN 2152-4955 doi: 10.1089/ten.tea.2011.0194

Abstract/Summary

The aim of this study was to construct an artificial fetal membrane (FM) by combination of human amniotic epithelial stem cells (hAESCs) and a mechanically enhanced collagen scaffold containing encapsulated human amniotic stromal fibroblasts (hASFs). Such a tissue-engineered FM may have the potential to plug structural defects in the amniotic sac after antenatal interventions, or to prevent preterm premature rupture of the FM. The hAESCs and hASFs were isolated from human fetal amniotic membrane (AM). Magnetic cell sorting was used to enrich the hAESCs by positive ATP-binding cassette G2 selection. We investigated the use of a laminin/fibronectin (1:1)-coated compressed collagen gel as a novel scaffold to support the growth of hAESCs. A type I collagen gel was dehydrated to form a material mimicking the mechanical properties and ultra-structure of human AM. hAESCs successfully adhered to and formed a monolayer upon the biomimetic collagen scaffold. The resulting artificial membrane shared a high degree of similarity in cell morphology, protein expression profiles, and structure to normal fetal AM. This study provides the first line of evidence that a compacted collagen gel containing hASFs could adequately support hAESCs adhesion and differentiation to a degree that is comparable to the normal human fetal AM in terms of structure and maintenance of cell phenotype.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/24314
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Electron Microscopy Laboratory (CAF) Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	Mary Ann Liebert, Inc.
Publisher Statement	This is a copy of an article published in the journal Tissue Engineering Part A © 2012[copyright Mary Ann Liebert, Inc.]; Tissue Engineering Part A is available online at: http://www.liebertonline.com
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