Combined neutron and X-ray diffraction studies of DNA in crystals and solutions

Leal, R. M. F., Callow, S., Callow, P., Blakeley, M. P., Cardin, C. J. ORCID: https://orcid.org/0000-0002-2556-9995, Denny, W. A., Teixeira, S. C. M., Mitchell, E. P. and Forsyth, V. T. (2010) Combined neutron and X-ray diffraction studies of DNA in crystals and solutions. Acta Crystallographica Section D Biological Crystallography, 66 (11). pp. 1244-1248. ISSN 1399-0047 doi: 10.1107/S0907444910017713

Abstract/Summary

Recent developments in instrumentation and facilities for sample preparation have resulted in sharply increased interest in the application of neutron diffraction. Of particular interest are combined approaches in which neutron methods are used in parallel with X-ray techniques. Two distinct examples are given. The first is a single-crystal study of an A-DNA structure formed by the oligonucleotide d(AGGGGCCCCT)2, showing evidence of unusual base protonation that is not visible by X-ray crystallography. The second is a solution scattering study of the interaction of a bisacridine derivative with the human telomeric sequence d(AGGGTTAGGGTTAGGGTTAGGG) and illustrates the differing effects of NaCl and KCl on this interaction.