In vitro evaluation of the prebiotic activity of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel

Mandalari, G., Palop, C.N., Tuohy, K., Gibson, G.R. ORCID: https://orcid.org/0000-0002-0566-0476, Bennett, R.N., Waldron, K.W., Bisignano, G., Narbad, A. and Faulds, C.B. (2007) In vitro evaluation of the prebiotic activity of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel. Applied Microbiology and Biotechnology, 73 (5). pp. 1173-1179. ISSN 0175-7598 doi: 10.1007/s00253-006-0561-9

Abstract/Summary

The prebiotic effect of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel was studied using pure and mixed cultures of human faecal bacteria. This was compared to the prebiotic effect of fructo-oligosaccharides (FOS). Individual species of bifidobacteria and lactobacilli responded positively to the addition of the bergamot extract, which contained oligosaccharides in the range of three to seven. Fermentation studies were also carried out in controlled pH batch mixed human faecal cultures and changes in gut bacterial groups were monitored over 24 h by fluorescent in situ hybridisation, a culture-independent microbial assessment. Addition of the bergamot oligosaccharides (BOS) resulted in a high increase in the number of bifidobacteria and lactobacilli, whereas the clostridial population decreased. A prebiotic index (PI) was calculated for both FOS and BOS after 10 and 24 h incubation. Generally, higher PI scores were obtained after 10 h incubation, with BOS showing a greater value (6.90) than FOS (6.12).

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/13171
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Uncontrolled Keywords	fructo-oligosaccharides, bergamot oligosaccharides, prebiotics, pectic, lactobacilli, bifidobacteria, OLIGONUCLEOTIDE PROBES, SITU HYBRIDIZATION, CHICORY INULIN, BY-PRODUCT, OLIGOFRUCTOSE, BACTERIA
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