The use of salts, buffers and surfactants in LAP-MALDI MS

Krenkel, H., Brown, J., Morris, M. and Cramer, R. ORCID: https://orcid.org/0000-0002-8037-2511 (2023) The use of salts, buffers and surfactants in LAP-MALDI MS. International Journal of Mass Spectrometry, 493. 117314. ISSN 1873-2798 doi: 10.1016/j.ijms.2023.117134

Abstract/Summary

Biological samples such as tissue extracts and enzymatic assays typically have a complex composition, which can interfere with analyte ionisation and detection in mass spectrometry (MS). Ionisation techniques such as electrospray ionisation (ESI) are often coupled online to an upfront chromatographic separation, whereas sample preparations for techniques such as conventional matrix-assisted laser desorption/ionisation (MALDI) are performed offline and, in the case of MALDI, rely on sample clean-up owing to different crystallisation behaviour. Liquid atmospheric pressure matrix-assisted laser desorption/ionisation (LAP-MALDI) MS is a hybrid ionisation technique that has been previously used to analyse a wide range of biological samples at fast acquisition rates. Here we report data from a systematic investigation of the influence of various buffer compounds, salts, surfactants, and other compounds necessary for biological sample preparation reflected in the signal intensity of a standard peptide mixture. Tricine showed the least signal reduction from the buffer compounds tested as did octyl-β-D-glucopyranoside for the surfactants. It can be concluded that LAP-MALDI MS can be used to analyse biological samples directly without major sample clean-up if their content of additives is not too high.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/113177
Identification Number/DOI	10.1016/j.ijms.2023.117134
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	Elsevier
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