Staphylococcus aureus FadB is a dehydrogenase that mediates cholate resistance and survival under human colonic conditions

Alsultan, A., Walton, G. ORCID: https://orcid.org/0000-0001-5426-5635, Andrews, S. C. ORCID: https://orcid.org/0000-0003-4295-2686 and Clarke, S. R. (2023) Staphylococcus aureus FadB is a dehydrogenase that mediates cholate resistance and survival under human colonic conditions. Microbiology, 169 (3). 001314. ISSN 1465-2080 doi: 10.1099/mic.0.001314

Abstract/Summary

Staphylococcus aureus is a common colonizer of the human gut and in doing so it must be able to resist the actions of the host’s innate defences. Bile salts are a class of molecules that possess potent antibacterial activity that control growth. Bacteria that colonize and survive in that niche must be able to resist the action of bile salts, but the mechanisms by which S. aureus does so are poorly understood. Here we show that FadB is a bile-induced oxidoreductase which mediates bile salt resistance and when heterologously expressed in Escherichia coli renders them resistant. Deletion of fadB attenuated survival of S. aureus in a model of the human distal colon.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/111458
Identification Number/DOI	10.1099/mic.0.001314
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	Microbiology Society
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