In vivo applications of bioorthogonal reactions- chemistry and targeting mechanisms

Mitry, M. M. A., Greco, F. and Osborn, H. M. I. ORCID: https://orcid.org/0000-0002-0683-0457 (2023) In vivo applications of bioorthogonal reactions- chemistry and targeting mechanisms. Chemistry - A European Journal, 29 (20). e202203942. ISSN 0947-6539 doi: 10.1002/chem.202203942

Abstract/Summary

Bioorthogonal chemistry involves selective biocompatible reactions between functional groups that are not normally present in biology. It has been used to probe biomolecules in living systems, and has advanced biomedical strategies such as diagnostics and therapeutics. In this review, challenges and opportunities encountered when translating in vitro biorthogonal approaches to in vivo settings are presented with a focus on methods to deliver the bioorthogonal reaction components. These methods include metabolic bioengineering, active targeting, passive targeting, and simultaneously used strategies. The suitability of bioorthogonal ligation reactions and bond cleavage reactions for in vivo applications is critically appraised, and practical considerations such as the optimum scheduling regimen in pretargeting approach are discussed. Finally, we present our own perspectives for this area and identify what, in our view, are the key challenges that must be overcome to maximize the impact of these approaches.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/109958
Identification Number/DOI	10.1002/chem.202203942
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Medicinal Chemistry Research Group Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
Publisher	Wiley
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